Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 64, Issue 8, Pages 1743-1746Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.64.1743
Keywords
peptide synthesis; glycopeptide; mucin-type; beta-galactosidase; sialyltransferase
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A sialyl T-antigen-linked tetrapeptide was prepared by the combined method of chemical synthesis and enzymatic synthesis. The GalNAc-linked peptide was first obtained bg using a commercial peptide synthesizer, and then a galactose residue was attached with beta-(1 --> 3)-linkage by transglycosylating with a recombinant beta-galactosidase from Bacillus circulans. The sialic acid residue was then combined by alpha-(2 --> 3)-linkage with sialytransferase from rat liver.
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