Journal
JOURNAL OF BACTERIOLOGY
Volume 182, Issue 15, Pages 4343-4347Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.15.4343-4347.2000
Keywords
-
Categories
Funding
- NIAMS NIH HHS [R01 AR044252, AR44542] Funding Source: Medline
- NIGMS NIH HHS [R01 GM051126, GM51126] Funding Source: Medline
Ask authors/readers for more resources
Previous attempts to introduce transposon Tn4001 into Mycoplasma pulmonis and Mycoplasma arthritidis have not been successful, possibly due to functional failure of the transposon's gentamicin resistance determinant. Tn4001C and Tn4001T were constructed, respectively, by insertion of a chloramphenicol acetyltransferase gene and the tetM tetracycline resistance determinant into Tn4001. Both Tn4001C and Tn4001T transposed in M. pulmonis, and Tn4001T transposed in M. arthritidis. The incorporation of a Tn4001T derivative that contained lacZ into either Mycoplasma species resulted in transformants with readily detectable LacZ activity. Tn4001T may be of general utility for use as a mycoplasma cloning vehicle because tetM functions in all species of Mycoplasma examined thus far.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available