Journal
ANNALS OF BOTANY
Volume 86, Issue 2, Pages 317-322Publisher
OXFORD UNIV PRESS
DOI: 10.1006/anbo.2000.1189
Keywords
evolution; phylogeny; Crocus vernus Hill (Iridaceae); in situ hybridization; chromosomal polymorphism; karyotype evolution; repetitive DNA
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The physical localization of three tandemly-organized repetitive DNA sequences was investigated by in situ hybridization to metaphase chromosomes of 11 Crocus vernus accessions. The sequences included were the 18S-25S rDNA, the 5S rDNA and a tandemly-repeated sequence cloned from C. vernus (clone pCvKB8). Ten 2n = 8 karyotypes from accessions ranging across the Alps and the Pyrenees could be interpreted as variations of a standard karyotype. Polymorphisms were found involving size of the satellite chromosomes, extra 5S rDNA sites, and extensive differences in size and number of pCvKB8 loci. The 2n = 16 type did not correspond to any possible tetraploid derived from the 2n = 8 types. (C) 2000 Annals of Botany Company.
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