A neutralizing antibody Fab-influenza haemagglutinin complex with an unprecedented 2:1 stoichiometry:: characterization and crystallization

The haemagglutinin HA is a trimer of identical subunits and is the more abundant viral surface glycoprotein of the influenza virus. It is the target of antibodies that neutralize viral infectivity. Antibodies that bind to HA with 3:1 and 1:1 stoichiometries have been identified. Here, an antibody whose Fab binds to HA with an unprecedented 2:1 Fab:HA stoichiometry is characterized. The complex has been crystallized and synchrotron data to 3.5 Angstrom resolution have been collected. Molecular replacement confirms the stoichiometry of the complex.