Application of high-performance liquid chromatography to the investigation of free radical reactions in biological systems

The determination of free radicals and their reaction products in biological systems is discussed, with an emphasis on the use of liquid chromatographic methods for the analysis. Free radicals formed from oxygen and its reduction products, nitric oxide, and lipid radicals are considered. Whilst reversed-phase high-performance liquid chromatography has found wide application for these analyses, ion-exclusion chromatography also has significant potential and some developments in this area are described. (C) 2000 Elsevier Science B.V. All rights reserved.