Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 48, Issue 8, Pages 3305-3310Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf0001278
Keywords
barley; beta-glucan; MALDI-MS; oligosaccharides; quantification
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This study is the first to apply matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to both qualitative and quantitative analyses of oligosaccharides of lichenase-hydrolyzed water-soluble beta-glucan from barley. Compared to high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector, MALDI-MS is a rapid technique with high accuracy and sensitivity and could be used to assess primary structural features of water-soluble beta-glucan from different barley varieties.
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