Journal
BIOTECHNOLOGY LETTERS
Volume 22, Issue 16, Pages 1311-1317Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1005694731039
Keywords
Bacillus subtilis; fed-batch cultivation; phosphate-starvation; phytase; plasmid
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A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).
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