Journal
JOURNAL OF PERIODONTOLOGY
Volume 71, Issue 8, Pages 1315-1318Publisher
AMER ACAD PERIODONTOLOGY
DOI: 10.1902/jop.2000.71.8.1315
Keywords
cytokines; DNA, analysis; interleukin-1; mouthwashes; polymorphisms, genetic
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Background: We describe a simple, non-invasive mouthwash sampling method for rapid DNA isolation to detect cytokine gene polymorphisms. In the present paper, interleukin-1 beta (IL-1B) and interleukin-1 receptor antagonist (IL-1RN) gene polymorphisms were studied. Methods: Two mouthwash samples and blood samples were collected from 11 healthy individuals. The second mouthwash sample was stored for 7 days at room temperature. Polymerase chain reaction amplification was used to identify a bi-allelic polymorphism at position +3953 in the IL-1B gene and a variable number of tandem repeats (VNTR) polymorphism in the IL-1RN gene. Results: Our results show that the typing of these cytokine gene polymorphisms using DNA isolated from mouthwash samples did not differ from those obtained by a phenol/chloroform isolation method from EDTA anti-coagulated blood. Moreover, reliable results from mouthwash samples were obtained after storage for at least 7 days at room temperature. Conclusions: Mouthwash can be the method of choice to study gene polymorphisms in periodontitis and other chronic inflammatory diseases.
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