Journal
HUMAN REPRODUCTION
Volume 15, Issue 8, Pages 1717-1722Publisher
OXFORD UNIV PRESS
DOI: 10.1093/humrep/15.8.1717
Keywords
chromatin structure; human fertility; in-vitro fertilization; intracytoplasmic sperm injection; SCSA
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The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pa-induced denaturation in situ. The mean percentage of spermatozoa in the neat sample demonstrating DNA denaturation was significantly lower in the seven men that initiated a pregnancy (15.4 +/- 4.6, P = 0.01) than in the 14 men who did not initiate a pregnancy (31.1 +/- 3.2). No pregnancies resulted if greater than or equal to 27% of the spermatozoa in the neat semen sample showed DNA denaturation. These data demonstrate that SCSA parameters are independent of conventional semen parameters. Furthermore, the SCSA may anew physicians to identify male patients for whom IVF and ICSI will be unlikely to result in pregnancy initiation.
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