Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 25, Issue 2, Pages 81-85Publisher
SPRINGER HEIDELBERG
DOI: 10.1038/sj.jim.7000033
Keywords
adhesion; biofilm; exopolysaccharide; Hyphomonas
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Biofilm formation commences with the adhesion of microorganisms to surfaces. Information regarding the initial bond between a bacterium and a solid surface is essential for devising methods to inhibit the onset of biofilm formation. Three different types of polysaccharide-specific probes, cationic metals, dyes, and lectins, were used to bind the exopolysaccharide of Hyphomonas rosenbergii, a budding, prosthecate marine bacterium. Probes, which specifically bind complex carbohydrates, inhibit the adhesion of H, rosenbergii to hydrophilic surfaces. These results suggest that the polysaccharide portion of H. rosenbergii capsular, extracellular polymeric-substance is involved in the primary adhesion process.
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