Journal
NUCLEIC ACIDS RESEARCH
Volume 28, Issue 15, Pages 2862-2865Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/28.15.2862
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Funding
- NCI NIH HHS [P30 CA042014, 2P30 CA42014-12] Funding Source: Medline
- NIGMS NIH HHS [R01 GM048152, GM 48152] Funding Source: Medline
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Design of antisense oligonucleotides targeting any mRNA can be much more efficient when several activity-enhancing motifs are included and activity-decreasing motifs are avoided, This conclusion was made after statistical analysis of data collected from >1000 experiments with phosphorothioate-modified oligonucleotides. Highly significant positive correlation between the presence of motifs CCAC, TCCC, ACTC, GCCA and CTCT in the oligonucleotide and its antisense efficiency was demonstrated, In addition, negative correlation was revealed for the motifs GGGG, ACTG, AAA and TAA, It was found that the likelihood of activity of an oligonucleotide against a desired mRNA target is sequence motif content dependent.
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