Journal
BIOTECHNOLOGY LETTERS
Volume 22, Issue 15, Pages 1217-1219Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1005602911977
Keywords
Fourier transform infrared spectroscopy; poly-beta-hydroxybutyrate
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An alternative method for the analysis of poly-beta-hydroxybutyrate (PHB) in microbial biomass based on FTIR spectroscopy was developed in which a semi-transparent, thin pellet of biomass was made with KBr and scanned by IR spectroscopy, from 400 cm(-1) to 2000 cm(-1). The IR spectra of the target biomass showed a distinct absorbance band of the carboxylic ester group at 1724.03 cm(-1). The technique can be used to screen large numbers of microorganisms for PHB production.
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