Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 11, Issue 4, Pages 325-330Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00107-5
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By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis, Recent advances include the evolution of a novel catalytic activity using the alp barrel scaffold, evolution of a cofactor-free monooxygenase, and the engineering of regulatable enzymes. New screening systems for enantioselectivity and protein solubility, and the continuing stream of new methods for creating enzyme libraries further extend evolution's reach.
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