4.5 Article

Two estrogen receptors expressed in the teleost fish, Sparus aurata:: cDNA cloning, characterization and tissue distribution

Journal

JOURNAL OF ENDOCRINOLOGY
Volume 166, Issue 2, Pages 293-306

Publisher

SOC ENDOCRINOLOGY
DOI: 10.1677/joe.0.1660293

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Estrogen is an essential hormone for many reproductive and non-reproductive functions. The function of estrogen in the reproductive cycle of seabream (Sparus aurata), a protandrous hermaphrodite teleost fish, is complex but it is understood to be involved in sex inversion, a process that occurs in some individuals during the second reproductive season. Estrogen action is mediated by two estrogen receptor (ER) subtypes designated alpha and beta. As a step to understanding the mechanisms of estrogen action during natural and induced sex reversal in seabream, we have isolated two cDNAs encoding distinct forms of ER homologous to mammalian ER alpha and ER beta. The seabream ER alpha clone (sbER alpha 1), which was truncated in the A/B domain, corresponded to a variant differing in five amino acids from another recently cloned sbER alpha. The ER beta clone (sbER beta) encoded a protein 559 amino acids long and showed only 40% identity to sbER alpha. Northern blot analysis of liver and ovary mRNA indicated the presence of several transcripts of the two receptor subtypes. PCR analysis showed that the two receptors differed in their expression pattern. sbER alpha had a more restricted distribution, occurring mainly in testis, liver and heart, and sbER beta was present in most tissues, being more abundant ill ovary, testis, liver, intestine and kidney. The presence in seabream of two ERs with several ER transcripts and their pattern of distribution are consistent with the widespread effects of estrogen in different tissues.

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