Journal
PHARMACOGENETICS
Volume 10, Issue 6, Pages 557-565Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00008571-200008000-00009
Keywords
cancer; pharmacogenetics; ultraviolet-sensitivity; recombination; polymorphism
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Glutathione S-transferase theta enzyme activity involved in the metabolism of toxic compounds is absent in approximately 20% of Caucasians due to a homozygous deletion of GSTT1 (*O/O), Because the exact manner of the GSTT1 deletion was unknown, current genotyping of GSTT1 was limited to detect the presence versus complete absence of the gene by a GSTT1-specific polymerase chain reaction (PCR). Thus, heterozygous (*A/O) and homozygous (*A/A) samples could not be discriminated. We have characterized the boundaries of the deletion of the human glutathione S-transferase theta (GSTT1) gene: PCR mapping and sequencing revealed a 54251 bp fragment including GSTT1 to be deleted from chromosome 22, most likely by a homologous recombination event between two highly homologous sequence stretches that flank GSTT1, Based on the knowledge of the GSTT1*O region, a PCR assay was devised for unambiguous discrimination of homozygously deleted (*O/O), heterozygously (*A/O) and homozygously GSTT1 carrying (*A/A) individuals. Genotyping of 180 samples of a Caucasian population revealed that the deletion consists of one defined allele, whose distribution in the population fits the Hardy-Weinberg equilibrium with observed 20% *O/O, 46% *A/O and 34% *A/A individuals. The number of GSTT1*A alleles detected by this procedure correlated highly significant with the enzyme activity in erythrocytes. Genotype-phenotype comparisons demonstrated a codominant type of inheritance by a gene-dose effect: samples with two active alleles expressed a statistically significant higher enzymatic activity compared to those with one null allele (P < 0.0001, ANOVA). Pharmacogenetics 10:557-565 (C) 2000 Lippincott Williams & Wilkins.
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