Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 27, Issue 3-5, Pages 212-218Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(00)00135-6
Keywords
Arthrobacter ureafaciens K-2032; DFA IV; levan fructotransferase; purification
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A microorganism producing levan fructotransferase was isolated from sugar-disclosed soil and it was identified as Arthrobacter ureafaciens. The major product from levan by enzyme reaction was identified as di-D-fructofuranose 2,6':6,2' dianhydride by mass spectrometry. nuclear magnetic resonance, and chemical analyses. Small amounts of several oligosaccharides and free fructose were also formed by enzyme reaction. An extracellular enzyme that produces di-D-fructofuranose 2,6':6,2' dianhydride from levan was purified from the culture broth of A. ureafaciens K2032. The enzyme had optimum activity around pH 5.8 and 45 degrees C and had a dimeric form in solution. The N-terminal amino acid residues of the purified enzyme were SAPGSLRAVYYHMTPPSGXLXDPQ. The enzyme has narrow substrate range and converts the levan to di-D-fructofuranose 2,6':6,2' dianhydride with around 62.5% conversion yield. (C) 2000 Elsevier Science Inc. All rights reserved.
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