Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 31, Issue 2, Pages 105-109Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2672.2000.00797.x
Keywords
-
Categories
Ask authors/readers for more resources
Multiplex PCR amplification of invA and virA genes was developed enabling simultaneous detection in mussels of Salmonella spp, and Shigella spp., respectively. Simultaneous amplification of products of 215 and 275 bp was obtained either by using mixtures of individual strains of Sh. dysenteriae and Salm. typhimurium or spiked cont aminated mussels with both bacteria. In the case of the mussels, 10-100 cells of Salmonella spp. and Shigella per millilitre of homogenate were detected by the multiples PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of cultivable bacteria. Also, the sensitivity and specificity of this method was evaluated. Multiplex PCR amplification was shown to be an effective, sensitive and rapid method for the simultaneous detection of pathogens in mussels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available