Journal
JOURNAL OF CELL BIOLOGY
Volume 150, Issue 3, Pages 681-688Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.150.3.681
Keywords
dynein; dynactin; lissencephaly; nudF; LIS1
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Funding
- NIGMS NIH HHS [R01 GM052309, 5RO1GM52309-03] Funding Source: Medline
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The nudF gene of the filamentous fungus Aspergillus nidulans acts in the cytoplasmic dynein/dynactin pathway and is required for distribution of nuclei. NUDE protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. Haploinsufficiency of the LIS1 gene causes a malformation of the human brain known as lissencephaly. We screened for multicopy suppressors of a mutation in the nudF gene. The product of the nudE gene isolated in the screen, NUDE, is a homologue of the nuclear distribution protein RO11 of Neurospora crassa. The highly conserved NH2-terminal coiled-coil domain of the NUDE protein suffices for protein function when overexpressed. A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 (MP43) of X. laevis. NUDF protein interacts with the Aspeugillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. In addition, NUDF coprecipitates with an epitope-tagged NUDE. The fact that NUDE and LIS1 interact with the same protein domain strengthens the notion that these two proteins are functionally related.
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