Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 45, Issue 1, Pages 57-63Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-022X(00)00098-1
Keywords
laccase; laccase activity; microcalorimetry; substrate
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The reactions between Laccase (extracted from China lacquer of Rhus vernicifera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogallol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry system. Based on calorimetry, a new method has been proposed. Laccase activity and the Michaelis constant K-m have been determined simultaneously by this method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying other enzymes catalyzing reactions using this method. (C) 2000 Elsevier Science B.V. All rights reserved.
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