Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 301, Issue 4, Pages 817-826Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/jmbi.2000.4022
Keywords
ribozyme; gene targeting; RNase P; herpes simplex virus; antiviral
Categories
Funding
- NIAID NIH HHS [AI41927] Funding Source: Medline
- NIGMS NIH HHS [GM54875] Funding Source: Medline
Ask authors/readers for more resources
A sequence-specific ribozyme (M1GS RNA) derived from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the mRNA encoding human herpes simplex virus 1 (HSV-1) major transcription activator, ICP4. A reduction of more than 80% in the expression level of ICP4 and a reduction of about 1000-fold in viral growth were observed in cells that stably expressed the ribozyme. Ln contrast, a reduction of less than 10% in ICP4 expression and viral growth was observed in cells that either did not express the ribozyme or produced a catalytically inactive ribozyme mutant. Thus, M1GS ribozyme is highly effective in inhibiting HSV-1 growth and can be used as a general gene-targeting agent for anti-HSV applications. (C) 2000 Academic Press.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available