Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 68, Issue 1-3, Pages 109-114Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/S0925-4005(00)00469-X
Keywords
lactate; lactate oxidase; sol-gel; enzyme stability
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Lactate oxidase (LOx) is an unstable enzyme. In this work, a variety of immobilisation techniques are investigated in an effort to improve the long-term stability of the enzyme. These include covalent linkage to two membrane types, encapsulation in a BSA gel and four different sol-gel matrices. The enzyme glucose oxidase (GOx) was also immobilised in the same sol-gel matrices. The methods were assessed for both activity and stability of the enzyme and the mechanical rigidity of the matrix. The BSA and sol-gels both formed physically robust enzyme layers. The enzyme retained its activity in the BSA gel for 20 days. Activity of the enzyme was much higher in the sol-gel matrices and remained stable fur at least 55 days. Sol-gel processing conditions were also investigated. (C) 2000 Elsevier Science S.A. All lights reserved.
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