Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 275, Issue 2, Pages 690-695Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.3348
Keywords
eicosanoid; 12(S)-HETE; PI3 kinase; PKC; ERK; MAPK; migration
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Funding
- NCI NIH HHS [CA 29997] Funding Source: Medline
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The arachidonic acid metabolite of 12 lipoxygenase, 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) promotes metastatic behavior of tumor cells. In this study we set out to identify 12(S)-HETE signaling pathways, and their contribution to cellular functions in A431 epidermoid carcinoma. (1) 12(S)-HETE stimulated phosphotyrosine associated PI3 kinase activity. (2) 12(S)-HETE stimulated ERK1/2 in a PI3 kinase dependent manner. (3) PI3 kinase affected the 12(S)-HETE stimulated Raf/MEK/ERK cascade at the level of MEK, (4) 12(S)-HETE stimulated ERK1/2 via PKC zeta. (5) 12(S)-HETE stimulated cell migration on laminin, which was eliminated by PI3 kinase and cPKC inhibitors, but it was unaffected by inhibition of ERK1/2. (C) 2000 Academic Press.
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