Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 275, Issue 2, Pages 637-642Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.3346
Keywords
inflammation; ion channels; smooth muscle; colon
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Funding
- NIDDK NIH HHS [DK46467] Funding Source: Medline
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We have investigated the activity of calcium and potassium channels in a murine model of experimental colitis, Colonic myocytes from dextran sulphate sodium (DSS)-treated mice were examined by whole cell patch clamp techniques. Myeloperoxidase activity was enhanced 3.5-fold in DSS-treated mouse colon. In whole cell voltage clamp, depolarization predominantly evoked net transient outward currents in DSS-treated mice and inward Ca2+ currents in control myocytes. Voltage-dependent L-type Ca2+ currents were studied using intracellular Cs+ in the patch pipette. Inward Ca2+ currents were markedly suppressed in inflamed colon. The peak currents at +10 mV depolarization were -3.93 +/- 0.88 pA/pF in control (n = 12) and -1.14 +/- 0.19 (n = 10) in DSS mice. In contrast there was no change in the amplitude, kinetics, or steady-state inactivation properties of the transient outward currents in control or DSS-treated colonic myocytes. Inflammation significantly enhanced activation of the ATP-sensitive K+ channel. At a holding potential of -50 mV, the K-ATP channel opener lemakalim induced an inward current of 2.02 +/- 0.5 pA/pF in control (n = 20) and 4.19 +/- 1.17 pA/pF in DSS-treated colon. These currents were abolished by glibenclamide, The present results suggest that inflammation of the colon results in selective changes in ion channel activity of smooth muscle cells. (C) 2000 Academic Press.
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