Journal
BIOLOGICAL CONTROL
Volume 66, Issue 3, Pages 204-208Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.biocontrol.2013.06.001
Keywords
Antifungal activity; Biological control agent; Crude extracts; Fusarium oxysporum f. sp cubense race 4; Streptomyces griseus; Wilt disease
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Funding
- Monash University Sunway Campus
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The antifungal activity of viable cells of Streptomyces griseus (St 4) and its cell-free extracts were investigated against the pathogenic Fusarium oxysporum f. sp. cubense race 4 (FOC race 4), causal agent of wilt disease in bananas. Results from in vitro and soil assays showed cells and cell-free extracts of S. griseus were able to inhibit FOC race 4 with varying degree of success. Antifungal activity was attributed to chitinase and beta-1,3-glucanase, detected in both cells and cell-free extracts, which caused lysis of fungal cell wall and inhibited sporulation. Interestingly, beta-1,3-glucanase and chitinase activities were significantly higher in cell-free extracts compared to cells, with 8.30 and 5.43 against 7.96 and 4.95 U mL(-1), respectively. Application to soil however, showed inoculation using S. griseus cells were more effective in suppressing growth of FOC race 4 than crude extracts, with 6 log(10) CFU of FOC race 4 g(-1) soil enumerated compared to 7 log(10) CFU of FOC race 4 g(-1) soil after 20 days. To summarize, this study has shown that cell-free extracts of S. griseus have antifungal properties but may not be suitable for soil application in its current form (liquid suspension). Further investigations on bioformulation may address this limitation. (C) 2013 Elsevier Inc. All rights reserved.
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