Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 18, Issue 1, Pages 65-68Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1008354229396
Keywords
bulk water; magnetization transfer; NMR screening; protein hydration; protein-ligand interactions
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A powerful screening by NMR methodology (WaterLOGSY), based on transfer of magnetization from bulk water, for the identification of compounds that interact with target biomolecules (proteins, RNA and DNA fragments) is described. The method exploits efficiently the large reservoir of H2O magnetization. The high sensitivity of the technique reduces the amount of biomolecule and ligands needed for the screening, which constitutes an important requirement for high throughput screening by NMR of large libraries of compounds. Application of the method to a compound mixture against the cyclin-dependent kinase 2 (cdk2) protein is presented.
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