Journal
JOURNAL OF BACTERIOLOGY
Volume 182, Issue 18, Pages 5267-5270Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.18.5267-5270.2000
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Funding
- NIAID NIH HHS [AI39615, AI07362, T32 AI007362] Funding Source: Medline
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Recently, M. Dmitrova et al. (Mol. Gen. Genet. 257:205-212, 1998) described a LexA-based genetic system to monitor protein-protein interactions in an Escherichia coli background. However, the plasmids used in this system, pMS604 and pDP804, were not readily amenable for general use. In this report, we describe modifications of both plasmids that allow fragments of DNA to be fused to either vector in any reading frame. Homodimerization and heterodimerization of full-length proteins involved in polysialic acid synthesis in E. coli gl, as well as heterodimerization between a full-length protein and a protein fragment, demonstrate the usefulness of the modified plasmids for investigating bacterial protein-protein interactions in vivo.
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