Journal
BIOLOGICAL CONTROL
Volume 54, Issue 3, Pages 197-205Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.biocontrol.2010.05.009
Keywords
Selective medium; Entomopathogenic fungi; Beauveria bassiana; Beauveria brongniartii; Metarhizium brunneum; Metarhizium acridum; Chloramphenicol; Thiabendazole; Cycloheximide
Categories
Funding
- Utah Department of Agriculture and Food [8173]
- United States Department of Agriculture (USDA, APHIS)
- Community/University Research Initiative of Utah State University
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The selective media most commonly used for isolating hyphomycetous species of entomopathogenic fungi from non-sterile substrates rely on N-dodecylguanidine monoacetate (dodine) as the selective fungicide. Although these media are effective for isolating many species of Metarhizium and Beauveria from soil, they are inefficient media for isolation of an important Metarhizium species, Metarhizium acridum, from non-sterile soil. Our current study was directed to formulating a dodine-free selective medium that is efficient for isolating naturally occurring Beauveria spp. and Metarhizium spp., especially M. acridum, from soil. The selective medium (designated CTC medium) consists of potato dextrose agar plus yeast extract (PDAY) supplemented with chloramphenicol, thiabendazole and cycloheximide. In comparisons with selective media previously reported in the literature, the CTC medium afforded colonies that were larger and had both earlier and more abundant conidiation of entomopathogenic fungi, features which greatly facilitated identification of the emerging entomopathogenic fungi. In addition to efficient re-isolation of M. acridum, this medium also is an effective tool for selective isolation of Metarhizium brunneum, Metarhizium robertsii, Beauveria bassiana and Beauveria brongniartii from non-sterile field-collected soil samples inoculated (spiked) with fresh conidia in the laboratory. (C) 2010 Elsevier Inc. All rights reserved.
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