Journal
TRENDS IN BIOTECHNOLOGY
Volume 18, Issue 9, Pages 380-388Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/S0167-7799(00)01475-X
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Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines. Owing to the commercial interest in these approaches, the development of production and purification strategies for gene-therapy vectors has been performed in pharmaceutical companies within a confidential environment. Consequently, the information on large-scale plasmid purification is scarce and usually not available to the scientific community. This article reviews downstream operations for the large-scale purification of plasmid DNA, describing their principles and the strategy used to attain a final product that meets specifications.
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