Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 891, Issue 1, Pages 109-114Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)00623-3
Keywords
vitamins; fat-soluble; provitamins
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The fat-soluble vitamins A, D-2, D-3, E and K-1, retinyl acetate, retinyl palmitate, tocopherol acetate, ergosterol and 7-dehydrocholesterol in milk were separated on a packed reversed-phase fused-silica microcolumn, and determined with UV detection. Conventional liquid chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated in gradient mode with detection limits comprised between 0.02 ng/ml for retinol and 2 ng/ml for vitamin E. All vitamins were separated in less than 17 min. Recovery studies showed good results for all solutes (89-107%) and the intra-day coefficients of variations ranged from 2 to 8%. Application of the proposed method to the quantification of fat-soluble vitamins in milk is reported. (C) 2000 Elsevier Science B.V. All rights reserved.
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