Journal
INFECTION AND IMMUNITY
Volume 68, Issue 9, Pages 5450-5453Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.68.9.5450-5453.2000
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Funding
- NHLBI NIH HHS [HL-55660] Funding Source: Medline
- NIAID NIH HHS [AI-16242, AI-39067, R29 AI040262] Funding Source: Medline
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Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.
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