Journal
JOURNAL OF CELL BIOLOGY
Volume 150, Issue 5, Pages 949-961Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.150.5.949
Keywords
subnuclear compartments; transcription; cell cycle; MCF7 differentiation; actinomycin D
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The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis, Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug-induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as a-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.
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