Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 10, Issue 1-3, Pages 107-115Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1381-1177(00)00118-1
Keywords
threonine aldolase; beta-hydroxy-alpha-amino acid; enzymatic resolution; stereoselective synthesis
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Threonine aldolase catalyzes the reversible interconversion of certain beta-hydroxy-alpha-amino acids and glycine plus the corresponding aldehydes. Various microbial threonine aldolases with different stereospecificities were found on extensive screening, and the genes encoding the proteins were cloned and heterogeneously overexpressed in Escherichia coli. By using recombinant threonine aldolases, an enzymatic resolution process was established for the production of optically pure P-hydroxy-a-amino acids. In addition, the threonine aldolase-catalyzed direct synthesis of beta-hydroxy-alpha-amino acid from aldehyde and glycine is discussed. (C) 2000 Elsevier Science B.V. Al rights reserved.
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