Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume 1493, Issue 1-2, Pages 151-158Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-4781(00)00176-7
Keywords
DNA polarisability; dielectric spectroscopy; counterion fluctuation
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The dielectric properties of 12 kbp plasmid DNA have been measured as a function of temperature in the range 5 degrees C to 40 degrees C. Time domain reflectometry was used to obtain dielectric data over the frequency range from 200 kHz to 3 GHz. Values of the frequency dependent polarisability per DNA macromolecule have been determined from the measurements. Possible mechanisms that could account for the dielectric dispersion are also discussed, in particular the counterion fluctuation model of Manning-Mandel-Oosawa. (C) 2000 Elsevier Science B.V. All rights reserved.
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