Interaction of human Ku70 with TRF2

Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DIVA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres, We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80, Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2, The transfected TFR2 colocalized with Ku70, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. all rights reserved.