Journal
PLANT SCIENCE
Volume 158, Issue 1-2, Pages 129-137Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-9452(00)00313-7
Keywords
Lilium formosanum; Wallace; long-term culture; regeneration ability; shoot bud; somatic embryo; 2,3,5-triiodobenzoic acid
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Cell suspension cultures of Lilium formosanum Wallace were initiated from bulb scale-derived calli and subcultured every 2 weeks using a medium containing 5 mu M 4-amino-3,5,6-trichloropicolinic acid (picloram). Almost all cell clumps from the suspension cultures developed numerous somatic embryos following their transfer onto a plant growth regulator-free medium, while they vigorously produced shoot buds on media containing 0.5 or 5 mu M 6-benzyladenine (BA). The high regeneration potential on a plant growth regulator-free medium was maintained for up to 54 months, but it gradually decreased thereafter, and only a few adventitious shoots and embryos were obtained from 75-month-old cultures. For restoring the regeneration potential of these cultures, various treatments with plant growth regulators were applied, among which about 10-fold increases in the number of regenerated shoot buds were obtained with 0.5 or 5 mu M 2,3,5-triiodobenzoic acid (TIBA) in combination with 0.5 or 5 mu M BA or N-(1,2,3-thiadiazol-5-yl)-N'-phenylurea (thidiazuron). Only shoot buds were produced from the cell clumps cultured on TIBA-containing media, and these shoot buds developed into complete plantlets after they were excised from the calli and transferred to a plant growth regulator-free medium. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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