Journal
FEBS LETTERS
Volume 481, Issue 2, Pages 169-176Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01927-X
Keywords
capsid-like particle; capsid-targeted viral inactivation; hepatitis B virus capsid; particulate vaccine carrier; Staphylococcus aureus nuclease
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The icosahedral nucleocapsid of hepatitis B virus (HBV) consists of multiple subunits of a single 183 amino acids (aa) core protein encasing the viral genome. However, recombinant core protein alone also forms capsid-like particles. We have recently shown that a 238 aa protein centrally inserted into the core protein can be displayed on the particle surface. Here we demonstrate that replacement of the C-terminal basic domain by the 17 kDa Staphylococcus aureus nuclease also yields particles but that in these the foreign domains are located in the interior. The packaged nuclease is enzymatically active, and the chimeric protein forms mosaic particles with the wild-type core protein. Hence the HBV capsid is useful as a molecular platform which, dependent on the fusion site, allows foreign protein domains to either be packaged into or be exposed on the exterior of the particle. These results are of relevance for the use of the HEV capsid as a vaccine carrier, and as a target for antiviral therapy. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
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