Journal
JOURNAL OF MEMBRANE BIOLOGY
Volume 177, Issue 2, Pages 117-128Publisher
SPRINGER
DOI: 10.1007/s002320001105
Keywords
sodium channels; local anesthetics; benzocaine; butamben; phospholipid membranes; H-2 NMR
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This paper presents experimental evidence that an aromatic compound that has a quadrupole moment locates in a polar headgroup region in the lipid membranes, but not in a membrane interior hydrophobic region, and discusses correlation to the site of action of benzocaine and butamben on sodium channels. The H-2 NMR spectra of benzocaine-d(4), benzocaine-d(5), butamben-d(4), and butamben-d(9) in the model membranes were observed. The H-2 NMR spectra of perdeuterated palmitic acid and selectively deuterated palmitic acids at C2, C3, C5, C6, C9, or C10, which were inserted into the lipid membranes, were also observed. The phosphatidylserine (PS), phosphatidylcholine (PC), and liquid mixtures composed of PS, PC, and phosphatidylethanolamine (PE), which contain or do not contain cholesterol, were employed. A moment analysis was applied to the H-2 NMR spectra of palmitic-d(31) acid. An order parameter, S-CD, for each carbon segment was calculated from the observed quadruple splitting. We concluded that in the lipid mixture containing cholesterol, the aromatic rings of benzocaine and butamben locate around the glycerol moiety of the lipids and that when there exists no cholesterol, they locate a little more inside from the headgroup region, directing, in both cases, their amino groups upward (polar region) and the ethyl and butyl groups downward (hydrophobic region). These data cast a question on the site of action of the neutral local anesthetics in the sodium channels.
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