Journal
JOURNAL OF IMMUNOLOGY
Volume 165, Issue 6, Pages 3358-3365Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.165.6.3358
Keywords
-
Categories
Ask authors/readers for more resources
Certain cationic antimicrobial peptides block the binding of LPS to LPS-binding protein and reduce the ability of LPS to induce the production of inflammatory mediators by macrophages. To gain a more complete understanding of how LPS activates macrophages and how cationic peptides influence this process, we have used gene array technology to profile gene expression patterns in macrophages treated with LPS in the presence or the absence of the insect-derived cationic antimicrobial peptide CEMA (cecropin-melittin hybrid). We found that CEMA selectively blocked LPS-induced gene expression in the RAW 264.7 macrophage cell line. The ability of LPS to induce the expression of >40 genes was strongly inhibited by CEMA, while LPS-induced expression of another 16 genes was relatively unaffected. In addition, CEMA itself induced the expression of a distinct set of 35 genes, including genes involved in cell adhesion and apoptosis, Thus, CEMA, a synthetic cu-helical peptide, selectively modulates the transcriptional response of macrophages to LPS and can alter gene expression in macrophages.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available