Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 111, Issue 1, Pages 216-222Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2141.2000.02341.x
Keywords
chronic myelogenous leukaemia; BCR-ABL rearrangement; fluorescence in situ hybridization; T cells; natural killer cells
Categories
Ask authors/readers for more resources
Chronic myelogenous leukaemia (CML) is a haematological malignant disorder characterized by the Philadelphia chromosome (Ph) and BCR-ABL gene rearrangement. This abnormal fusion gene can be considered to serve as a marker for the transformed cell clone in CML and is found in all cells arising from the same malignant precursor cell. It has been detected in CML cells of the myeloid, monocytic, erythroid and B-lymphocytic lineages. However, it is still arguable as to whether T lymphocytes or natural killer (NK) cells carry this marker Answering this question would clarify the ontogenic relationship between NE; cells and T cells. We examined 12 CML patients and studied the expression of BCR-ABL rearrangement by fluorescence in situ hybridization (FISH) in both NK cells and T cells sorted by flow cytometry. The purity of T cells was 95.6-99.8% and that of NK cells was 95.3-99.3% after sorting. Neither NK cells nor T cells showed any positive BCR-ABL signal with the exception of one patient who recovered from a lymphoid blastic crisis. We speculate that T cells and NK cells originate from BCR-ABL-negative stem cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available