Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 10, Pages 4510-4513Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.10.4510-4513.2000
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We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.
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