Optical fiber biosensor for the determination of low biochemical oxygen demand

An optical fiber biosensor was developed for the evaluation of low Biochemical Oxygen Demand (BOD) values in river waters. Artificial wastewater (AWW) solution was employed as standards for the calibration of the BOD sensor. The response time of the sensor was 15 min, and the optimal BOD response was observed at 30 degreesC, pH 7.0. A linear relationship was obtained between the output voltage and BOD, values, and the range of determination was 1-10 mg l(-1) BOD. The sensor response was almost not influenced by chloride ion up to 1000 mg l(-1), and also not affected by heavy metal ions (Fe(3+), Cu(2+), Mn(2+), Cr(3+), Zn(2+)). The BOD of river waters was estimated by using the optical fiber biosensor, and good correlation between the sensor and BOD, test was obtained (r(2) = 0.971). (C) 2000 Elsevier Science S.A. All rights reserved.