Journal
BIOLOGICAL CHEMISTRY
Volume 394, Issue 1, Pages 113-123Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2012-0202
Keywords
caspase activation; cell viability; MAP kinase activation; migration; primary keratinocytes; silver nanoparticles
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Funding
- European Community [COST action BM0903]
- Polish Ministry of Science and Higher Education [776/N-COST/2010/0]
- European Union [POIG.02.01.00-12-064/08]
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Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings, particularly in wound dressings but also in cosmetics. This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on primary normal human epidermal keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that AgNPs may present possible danger for primary keratinocytes, concerning activation of genotoxic and cytotoxic processes depending on the concentration.
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