4.6 Article

Rapid and simple determination of the Escherichia coli phylogenetic group

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 10, Pages 4555-4558

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.10.4555-4558.2000

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Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on tripler PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.

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