Journal
BIOLOGICAL CHEMISTRY
Volume 393, Issue 12, Pages 1477-1483Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2012-0269
Keywords
degradomics; N-terminomics; protease; proteomics; terminal amine isotopic labeling of substrates (TAILS); Trans-Proteomic Pipeline (TPP)
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Funding
- Canadian Institutes of Health Research
- Breast Cancer Metastases from the Canadian Breast Cancer Research Alliance
- Canadian Breast Cancer Foundation
- Cancer Research Society
- Canada Research Chair in Metalloproteinase Proteomics and Systems Biology
- European Commission
- Swiss National Science Foundation [31003A_140726/1]
- Swiss National Science Foundation (SNF) [31003A_140726] Funding Source: Swiss National Science Foundation (SNF)
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Data analysis in proteomics is complex and with the extra challenges involved in the interpretation of data from N-terminomics experiments, this can be daunting. Therefore, we have devised a rational pipeline of steps to approach N-terminomics data analysis in a statistically-based and valid manner. We have automated these steps in CLIPPER, an add-on to the Trans-Proteomic Pipeline (TPP). Applying CLIPPER to the analysis of N-terminomics data generated by terminal amine isotopic labeling of substrates (TAILS) enables high confidence peptide to protein assignment, protein N-terminal characterization and annotation, and for protease analysis readily allows protease substrate discovery with high confidence.
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