Journal
BIOLOGICAL CHEMISTRY
Volume 393, Issue 4, Pages 249-258Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2011-0245
Keywords
electrostatic repulsion hydrophilic liquid interaction chromatography; glycopeptide enrichment; glycoproteome; glycosylation; lectin; titanium dioxide
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Funding
- Ministerium fur Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen
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Glycosylations are ubiquitous and, in many cases, essential protein modifications. Yet comprehensive and detailed analysis of glycosylations on a proteome-wide scale is a daunting and still unsolved challenge. However, a common workflow has emerged over the last decade for large-scale N-glycosylation site annotation by application of proteomic methodology. Thereby, the qualitative and quantitative assessment of hundreds or thousands of modification sites is enabled. This review presents a short overview about common enrichment techniques and glycosylation site detection for N-glycopeptides, including benefits and challenges of analysis.
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