Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 11, Issue 10, Pages 916-920Publisher
AMER CHEMICAL SOC
DOI: 10.1016/S1044-0305(00)00168-9
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Funding
- NIGMS NIH HHS [GM47356] Funding Source: Medline
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A new method of identifying and quantifying the disaccharide building blocks of glycosaminoglycans is introduced. The polysaccharides are subjected to an enzymatic digestion that releases the sulfated disaccharides. The disaccharides are then identified using a combination of electrospray ionization mass spectrometry and tandem mass spectrometry. Quantification of the isomeric disaccharides is also achieved by tandem mass spectrometry, using a recently developed methodology which quantifies mixtures of isomers without the use of chromatography or prior separation. Using mass spectrometry to characterize the components of glycosaminoglycans significantly reduces both sample consumption and analysis time of traditional methods. (J Am Soc Mass Spectrom 2000, 11, 916-920) (C) 2000 American Society for Mass Spectrometry.
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