Journal
BIOLOGICAL CHEMISTRY
Volume 390, Issue 2, Pages 175-179Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/BC.2009.021
Keywords
animal disease models; enzyme kinetics; human cathepsin B; murine cathepsin B
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Funding
- Slovenian Ministry of Higher Education, Science and Technology [P0140, J1-6488]
- European Commission [LSHG-CT-2006-018830, 201279]
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Validation of drug targets and subsequent preclinical studies are usually carried out on animal disease models, with mouse being the most commonly used. However, results from mouse models cannot always be directly related to human disease. Major discrepancies between the properties of murine and human variants were observed during the evaluation of compounds targeting cathepsins S and K. It is important, therefore, to know whether similar differences exist between murine and human cathepsin B. Thus, both enzymes were expressed and biochemically characterized. The enzymes exhibited similar biochemical properties, indicating that cathepsin B transgenic mouse models could be useful for studying its role in human pathologies.
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