Imaging spatial and cellular characteristics of low temperature calcium signature after cold acclimation in Arabidopsis

Cooling-induced 'calcium signatures' were imaged in aequorin-expressing Arabidopsis plants after cold acclimation or growth at ambient temperature. In all tissues, signatures were altered after acclimation. Characterization of the components generating this response indicates that cold acclimation increases cold-induced vacuolar Ca(2+) release, but does not affect the influx of extracellular calcium.