Journal
BLOOD CELLS MOLECULES AND DISEASES
Volume 26, Issue 5, Pages 445-452Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bcmd.2000.0320
Keywords
zebrafish; hematopoiesis; virus; anemia; fish
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Funding
- NIDDK NIH HHS [5P50 DK49216-05, R01 DK53298-03] Funding Source: Medline
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The zebrafish Danio rerio is a new model system for studying the genetics of hematopoiesis. To define naturally occurring viruses which could infect and replicate within hematopoietic precursors of the zebrafish, infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) were studied. Infection of whole fish with viral supernatants demonstrated infectious replicants for both viruses, indicating that the virus host range includes the zebrafish. In other species, infection with these viruses leads to prominent hematopoietic necrosis of the head kidney, the major site of adult hematopoiesis. We detected a transient toxicity of the virus to hematopoietic precursors and terminally differentiated red cells after viral infections. The kinetics of hematopoietic defects between IHNV and IPNV infection differed; fish infected with either virus, however, recovered by 6 days postinfection. In contrast to other fish infected with the virus, hematocrit did not change appreciably during this time. These studies are the first to demonstrate MNV and IPNV infection of the zebrafish and reveal the potential for use of such viruses for gene transfer experiments to infect zebrafish hematopoietic cells. (C) 2000 Academic Press.
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