Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 64, Issue 10, Pages 2225-2228Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.64.2225
Keywords
osteopontin gene; involution; mouse mammary gland; dexamethasone
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To understand the molecular mechanisms of mammary gland involution, an involution-induced clone was identified from a cDNA library of mouse mammary gland by differential screening. Characterization of a clone by sequencing and northern analysis showed that expression of the osteopontin gene was induced during involution of mouse mammary gland. But induction of the osteopontin gene was not observed in apoptotic HC11 mammary epithelial cells under serum starvation. In HCII cells, dexamethasone treatment from the seeding stage showed five-fold induction of osteopontin gene expression, but the expression was not changed when dexamethasone was added to confluent cells.
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